phosphate method meaning in Chinese
磷酸盐法
Examples
- The optimal expressing time with the calcium phosphate method was 72h after transfection , and the transfection method of calcium phosphate is proved to be more effective than the lipofection method
结果表明，采硕士论文戴君勇： k亚科动物的亲缘关系及hsblys的真核表达用cos 7作为真核表达细胞株，其表达目的蛋白的最佳时间是72小时。 - 1 ( + ) / hsblys was trans fected into cos - 7 cells with the calcium phosphate method and the lipofection method . we cultured the recombined cos - 7 cells at optimal condition and harvested cells every 24h . in order to find out the optimal expressive condition , we lysed the cells , extracted the protein , and measure the content of the hsblys
此外，用构建的融合表达载体pcdna3 . 1 ( + ) hsblys转染真核宿主细胞cos - 7 ，然后在合适的培养条件下进行表达，取不同表达时间( 48h 、 72h和96h )的细胞破碎后进行分析鉴定，找出最合适的表达时间及其他条件。